Plant Transcription Factor Database
Previous version: v3.0
Catharanthus roseus
HSF Family
Species TF ID Description
cra_locus_11487_iso_1HSF family protein
cra_locus_11487_iso_4HSF family protein
cra_locus_15449_iso_1HSF family protein
cra_locus_16414_iso_1HSF family protein
cra_locus_20485_iso_2HSF family protein
cra_locus_22026_iso_1HSF family protein
cra_locus_22026_iso_3HSF family protein
cra_locus_23780_iso_1HSF family protein
cra_locus_23780_iso_4HSF family protein
cra_locus_2495_iso_1HSF family protein
cra_locus_25625_iso_1HSF family protein
cra_locus_2766_iso_1HSF family protein
cra_locus_2766_iso_2HSF family protein
cra_locus_3966_iso_1HSF family protein
cra_locus_3966_iso_3HSF family protein
cra_locus_3966_iso_4HSF family protein
cra_locus_42582_iso_1HSF family protein
cra_locus_43336_iso_1HSF family protein
cra_locus_5171_iso_2HSF family protein
cra_locus_5171_iso_3HSF family protein
cra_locus_54367_iso_1HSF family protein
cra_locus_5900_iso_1HSF family protein
cra_locus_5900_iso_5HSF family protein
cra_locus_5900_iso_6HSF family protein
cra_locus_5900_iso_7HSF family protein
cra_locus_6181_iso_1HSF family protein
cra_locus_6222_iso_1HSF family protein
cra_locus_6786_iso_3HSF family protein
cra_locus_7303_iso_1HSF family protein
cra_locus_7303_iso_2HSF family protein
cra_locus_7303_iso_3HSF family protein
cra_locus_7303_iso_4HSF family protein
cra_locus_7303_iso_5HSF family protein
cra_locus_80_iso_10HSF family protein
cra_locus_80_iso_1HSF family protein
cra_locus_80_iso_3HSF family protein
cra_locus_80_iso_4HSF family protein
cra_locus_80_iso_6HSF family protein
cra_locus_80_iso_7HSF family protein
cra_locus_80_iso_9HSF family protein
cra_locus_9088_iso_1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645